Purification and properties of O6-methylguanine-DNA transmethylase from rat liver.
نویسندگان
چکیده
منابع مشابه
Regulation of O6-methylguanine-DNA methyltransferase levels in rat liver and kidney.
It was found that rat kidney contains a protein similar to that previously described in rat liver which catalyzes the transfer of the methyl group from O6-methylguanine in DNA to a protein-bound cysteine residue. The amount of the renal O6-methylguanine-DNA methyltransferase was increased up to 2.5-fold during renal hypertrophy in response to unilateral nephrectomy or treatment with folic acid....
متن کاملRegulation of O6-Methylguanine-DNA Methyltransferase Levels in Rat Liver and Kidney1
It was found that rat kidney contains a protein similar to that previously described in rat liver which catalyzes the transfer of the methyl group from O6-methylguanine in DNA to a proteinbound cysteine residue. The amount of the renal O6-methylguanine-DNA methyltransferase was increased up to 2.5-fold during renal hypertrophy in response to unilateral nephrectomy or treatment with folie acid. ...
متن کاملDose- and cell cycle-dependent O6-methylguanine elimination from DNA in regenerating rat liver after [14C]dimethylnitrosamine injection.
To study relations between increased transformation sensi tivity during DMA synthesis and carcinogen-induced molecular DMA alterations in regenerating liver, the kinetics of formation and the persistence of alkylated DMA bases were determined 10 to 240 min after a single [14C]dimethylnitrosamine (DMN) injection (1.43 and 4.0 mg/kg) during G, (12 h after partial hepatectomy) and in synchronized ...
متن کاملPurification to apparent homogeneity and partial amino acid sequence of rat liver O6-alkylguanine-DNA-alkyltransferase
O6-alkylguanine-DNA-alkyltransferase (ATase) activity was increased in rat liver from 80 to 320 fmoles/mg total protein 48 h after administration of 2-acetylaminofluorene at 60 mg/kg body weight. This tissue was used as a source of ATase which was purified by ammonium sulphate precipitation and DNA-cellulose, molecular exclusion and ion exchange chromatography (IEC). IEC purified material showe...
متن کاملStability and capacity of dimethylnitrosamine-induced O6-methylguanine repair system in rat liver.
Repeated administration of dimethylnitrosamine (DMN) (2 mg/kg for 3 weeks) to BD IV rats results in an increased capacity for the liver to repair O6-methylguanine in DNA, whereas other DNA adducts (7-methylguanine and 3-methyladenine) are not affected. In the experiments reported here, data on the rapidity of action of the enhanced system, its capacity after different challenging doses of [14C]...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1983
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)32927-2